APPLICATION OF DNA TECHNIQUES FOR IDENTIFICATION OF FUNGAL COMMUNITIES COLONIZING BOOK OF EGYPT VOLUMES V PLANCHES ANTIQUITIES

Document Type : Original Article

Authors

1 Assoc. Prof. Heritage Resources Management & Tour Guidance dept., College of Tourism & Archaeology, King Saud Univ. Riyadh, KSA. Conservation department, faculty of archaeology, Cairo Univ.

2 Conservation dept., High Institute of Tourism, Hotel Management & Restoration, Alexandria.

Abstract

This study represents both a study case about the fungi associated with a description of
Egypt volumes V Planches Antiquities in the Central library of Islamic manuscripts in Cairo,
were used for identification of isolates from these brownish area from a description of Egypt
The plate method with a DNA was used for identification of fungi. The two internal transcribed
spacers and the 5.8S gene (ITS1-5.8S- ITS2) from the nuclear ribosomal DNA were
amplified. The results show that the most dominant fungi isolated from the examined old
paper samples belong to Aspergillus spp. (niger – flavus – fumigates and – terreus). In the
present study, culture-independent molecular methods were applied to identify fungi
communities colonizing paper samples. Some of these fungi are cellulolytic species. Pure
fungal strains were used to constitute a marker for further comparative investigations of a
description of Egypt volumes V Planches Antiquities.
This study represents both a study case about the fungi associated with a description of
Egypt volumes V Planches Antiquities in the Central library of Islamic manuscripts in Cairo,
were used for identification of isolates from these brownish area from a description of Egypt
The plate method with a DNA was used for identification of fungi. The two internal transcribed
spacers and the 5.8S gene (ITS1-5.8S- ITS2) from the nuclear ribosomal DNA were
amplified. The results show that the most dominant fungi isolated from the examined old
paper samples belong to Aspergillus spp. (niger – flavus – fumigates and – terreus). In the
present study, culture-independent molecular methods were applied to identify fungi
communities colonizing paper samples. Some of these fungi are cellulolytic species. Pure
fungal strains were used to constitute a marker for further comparative investigations of a
description of Egypt volumes V Planches Antiquities.

Keywords

Egyptian Journal of Archaeological and Restoration Studies
Volume 4, Issue 2
December 2014
Pages 105-112

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