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Egyptian Journal of Archaeological and Restoration Studies
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A., A., A., T. (2014). APPLICATION OF DNA TECHNIQUES FOR IDENTIFICATION OF FUNGAL COMMUNITIES COLONIZING BOOK OF EGYPT VOLUMES V PLANCHES ANTIQUITIES. Egyptian Journal of Archaeological and Restoration Studies, 4(2), 105-112. doi: 10.21608/ejars.2014.7265
Afandy A.; Taha A.. "APPLICATION OF DNA TECHNIQUES FOR IDENTIFICATION OF FUNGAL COMMUNITIES COLONIZING BOOK OF EGYPT VOLUMES V PLANCHES ANTIQUITIES". Egyptian Journal of Archaeological and Restoration Studies, 4, 2, 2014, 105-112. doi: 10.21608/ejars.2014.7265
A., A., A., T. (2014). 'APPLICATION OF DNA TECHNIQUES FOR IDENTIFICATION OF FUNGAL COMMUNITIES COLONIZING BOOK OF EGYPT VOLUMES V PLANCHES ANTIQUITIES', Egyptian Journal of Archaeological and Restoration Studies, 4(2), pp. 105-112. doi: 10.21608/ejars.2014.7265
A., A., A., T. APPLICATION OF DNA TECHNIQUES FOR IDENTIFICATION OF FUNGAL COMMUNITIES COLONIZING BOOK OF EGYPT VOLUMES V PLANCHES ANTIQUITIES. Egyptian Journal of Archaeological and Restoration Studies, 2014; 4(2): 105-112. doi: 10.21608/ejars.2014.7265

APPLICATION OF DNA TECHNIQUES FOR IDENTIFICATION OF FUNGAL COMMUNITIES COLONIZING BOOK OF EGYPT VOLUMES V PLANCHES ANTIQUITIES

Article 2, Volume 4, Issue 2, December 2014, Page 105-112  XML PDF (270.57 K)
Document Type: Original Article
DOI: 10.21608/ejars.2014.7265
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Authors
Afandy A.1; Taha A.2
1Assoc. Prof. Heritage Resources Management & Tour Guidance dept., College of Tourism & Archaeology, King Saud Univ. Riyadh, KSA. Conservation department, faculty of archaeology, Cairo Univ.
2Conservation dept., High Institute of Tourism, Hotel Management & Restoration, Alexandria.
Abstract
This study represents both a study case about the fungi associated with a description of
Egypt volumes V Planches Antiquities in the Central library of Islamic manuscripts in Cairo,
were used for identification of isolates from these brownish area from a description of Egypt
The plate method with a DNA was used for identification of fungi. The two internal transcribed
spacers and the 5.8S gene (ITS1-5.8S- ITS2) from the nuclear ribosomal DNA were
amplified. The results show that the most dominant fungi isolated from the examined old
paper samples belong to Aspergillus spp. (niger – flavus – fumigates and – terreus). In the
present study, culture-independent molecular methods were applied to identify fungi
communities colonizing paper samples. Some of these fungi are cellulolytic species. Pure
fungal strains were used to constitute a marker for further comparative investigations of a
description of Egypt volumes V Planches Antiquities.
This study represents both a study case about the fungi associated with a description of
Egypt volumes V Planches Antiquities in the Central library of Islamic manuscripts in Cairo,
were used for identification of isolates from these brownish area from a description of Egypt
The plate method with a DNA was used for identification of fungi. The two internal transcribed
spacers and the 5.8S gene (ITS1-5.8S- ITS2) from the nuclear ribosomal DNA were
amplified. The results show that the most dominant fungi isolated from the examined old
paper samples belong to Aspergillus spp. (niger – flavus – fumigates and – terreus). In the
present study, culture-independent molecular methods were applied to identify fungi
communities colonizing paper samples. Some of these fungi are cellulolytic species. Pure
fungal strains were used to constitute a marker for further comparative investigations of a
description of Egypt volumes V Planches Antiquities.
Keywords
manuscripts; DNA; Fungal strains; investigations; Diagnosis
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